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. 2008 Sep;148(1):620–641. doi: 10.1104/pp.108.123141

Table V.

Arabidopsis proteins whose abundance was significantly smaller in deteriorated seeds than in control seeds during germination sensu stricto (1 d after imbibition)

No.a Experimental Molecular Mass Experimental pI Arabidopsis Protein Name Theoretical Molecular Mass Theoretical pI AGI No. Coverage Relative Abundanceb T1/2
kD kD ratio 7 d:0 d d
Translation and protein metabolism
    128c 47.45 5.45 Elongation factor 1B-γ 46.66 5.36 At1g09640 26% <0.28 >7
    129c 47.20 5.50 Eukaryotic initiation factor 4A-1 46.70 5.47 At3g13920 28% <0.49 >7
    105c 48.63 5.61 Elongation factor 1-γ2 46.4 5.55 At1g57720 41% <0.5 >7
    314d 37.54 5.22 60S acidic ribosomal protein P0-C 34.37 4.78 At2g40010 7% <0.25 >7
    200c 40.57 5.72 Protein disulfide isomerase-like (PDIL) 39.50 5.80 At2g47470 20% <0.44 >7
    364d 65.22 5.56 T-complex protein 1, θ-subunit (TCP-1-θ) 58.92 5.01 At3g03960 2% <0.30 >7
Energy metabolism
    39c 38.52 6.23 Glyceraldehyde-3-P dehydrogenase 36.91 6.62 At3g04120 26% <0.48 >7
    40c 38.55 6.26 Glyceraldehyde-3-P dehydrogenase 36.91 6.62 At3g04120 27% <0.44 >7
    307c 40.30 5.87 Glyceraldehyde-3-P dehydrogenase 36.91 6.62 At3g04120 19% <0.21 >7
    365c 62.77 6.30 Isocitrate lyase 50.42 6.29 At3g21720 16% <0.21 >7
    126c 27.16 5.51 Triose phosphate isomerase, cytosolic 27.15 5.24 At3g55440 10% <0.36 >7
    353c 55.70 6.60 Phosphoglucomutase 63.44 5.73 At1g70730 6% <0.10 >7
    372c 41.08 5.71 Phosphoglycerate kinase 42.11 5.49 At1g79550 21% <0.35 >7
    194c 45.5 6.15 3-Oxoacyl-[acyl-carrier-protein] synthase I 50.41 8.29 At5g46290 44% <0.49 >7
Cell detoxification and stress response
    23c 56.48 6.64 Catalase 56.93 6.63 At4g35090 16% <0.25 >7
    146c 37.67 5.03 MST 41.89 5.95 At1g79230 35% <0.30 >7
    29c 38.35 5.72 2-Alkenal reductase 38.13 5.81 At5g16970 14% <0.26 >7
    284d 25.34 5.43 Reduced glutathione-dependent dehydroascorbate reductase 23.62 5.79 At1g19570 57% <0.21 >7
    94c 34.92 5.21 Late embryogenesis abundant (LEA) 31.31 5.21 At3g17520 21% <0.27 >7
Amino acid metabolism
    196c 41.46 6.57 Asp aminotransferase 44.3 6.8 At5g19550 28% <0.31 >7
    174c 38.7 5.73 O-Acetyl-Ser-thiol-lyase 33.8 5.9 At4g14880 9% <0.26 >7
    175c 37.85 5.71 O-Acetyl-Ser-thiol-lyase 33.8 5.9 At4g14880 29% <0.22 >7
Cell division
    24c 42.94 5.06 Actin 7 41.73 5.31 At5g09810 23% <0.43 >7
    4c 57.35 4.89 Tubulin β2β3 chain 50.73 4.70 At5g62700 35% <0.32 >7
Seed storage proteins
    504c 60.87 6.20 12S seed storage protein (precursor) 55.86 6.36 At4g28520 7% <0.18 >7
    311c 14.1 3.2 β-Cruciferin 12S (seed storage protein fragment) 58.23 6.53 At4g28520 11% <0.21 >7
Other processes
    182c 50.76 5.53 DEAD box RNA helicase 48.38 5.49 At5g11200 32% <0.42 >7
    361c 54.65 5.22 4-Methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein 41.84 5.08 At3g14990 6% <0.16 >7
    116c 30.24 5.77 Expressed protein 28.78 5.92 At5g45690 40% <0.35 >7
a

Protein numbering following Arabidopsis seed protein reference maps available at http://www.seed-proteome.com.

b

Data obtained from densitometric analysis of individual spots from proteins on 2D gels stained with silver nitrate (see Fig. 2A for an example of a 2D gel): normalized spot volume in the deteriorated seeds (7 d of CDT) incubated for 1 d in water divided by the normalized spot volume in the nondeteriorated control seeds (0 d of CDT) incubated for 1 d in water, from five different gels and three independent extractions.

c

Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005).

d

Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table S2.