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. 2008 Sep;148(1):620–641. doi: 10.1104/pp.108.123141

Table VI.

Arabidopsis proteins whose de novo synthesis was inhibited during germination sensu stricto (1 d after imbibition) of deteriorated seeds submitted to CDT for 2 d compared with nondeteriorated control seeds

No.a Experimental Molecular Mass Experimental pI Arabidopsis Protein Name Theoretical Molecular Mass Theoretical pI AGI No. Coverage
kD kD
Translation and protein metabolism
    127b 93.92 5.89 Elongation factor EF-2 94.25 5.89 At1g56070 24%
    9b 17.94 5.50 HSP17.6 17.83 5.22 At5g12030 14%
    45b 76.38 5.24 HSP70 70.91 5.30 At1g16030 24%
    137b 76.06 5.07 HSP70 71.37 5.03 At3g12580 19%
    190b 101.62 5.82 HSP101 101.27 5.99 At1g74310 16%
    364c 65.22 5.56 T-complex protein 1, θ-subunit (TCP-1-θ) 58.92 5.01 At3g03960 2%
    579b 36.05 3.98 Gly-rich protein similar to P23 cochaperone 25.47 4.17 At4g02450 24%
Energy metabolism
    17b 73.40 6.58 Phosphoenolpyruvate carboxykinase 73.40 6.61 At4g37870 17%
    197b 40.91 6.77 Fru-bisphosphate aldolase 38.37 7.46 At2g36460 23%
    198b 40.72 6.89 Fru-bisphosphate aldolase 38.37 7.46 At2g36460 41%
    39b 38.52 6.23 Glyceraldehyde-3-P dehydrogenase, cytosolic 36.99 6.34 At3g04120 26%
    40b 38.55 6.26 Glyceraldehyde-3-P dehydrogenase, cytosolic 36.99 6.34 At3g04120 27%
    365b 62.77 6.30 Isocitrate lyase 50.42 6.29 At3g21720 16%
    188b 96.69 5.83 Aconitate hydratase, cytoplasmic 98.13 6.36 At4g35830 10%
Cell detoxification and stress response
    11b 19.61 5.75 Em-like protein GEA1 16.61 5.75 At3g51810 26%
    61b 66.68 5.78 Late embryogenesis abundant (LEA) 67.19 5.78 At2g42560 43%
    91b 61.88 5.06 Late embryogenesis abundant (LEA) 52.08 5.29 At3g53040 17%
    102b 60.51 5.22 Late embryogenesis abundant (LEA) 48.49 5.43 At2g36640 16%
    210b 88.93 4.93 Low-temperature-induced 65-kD protein 65.95 4.81 At5g52300 20%
    146b 37.67 5.03 MST 41.89 5.95 At1g79230 35%
    13b 72.97 6.30 MLO-like protein 67.21 10.06 At1g61560 15%
    254b 17.96 5.20 Dehydrin 18.44 7.95 At5g66400 6%
    255b 17.96 5.37 Dehydrin 18.44 7.95 At5g66401 6%
DNA metabolism
    186b 96.47 5.78 DNA topoisomerase I 102.78 10.05 At5g55300 8%
    187b 96.47 5.81 DNA topoisomerase I 102.78 10.05 At5g55300 8%
Amino acid metabolism
    174b 38.71 5.73 O-Acetyl-Ser-thiol-lyase 33.79 5.95 At4g14880 9%
    206b 82.05 6.09 5-Methyl-tetra-hydropteroyl-tri-Glu-homo-Cys methyltransferase 84.34 6.47 At5g17920 17%
Hydrolase and protease
    67b 63.25 6.47 Glycosyl hydrolase family 1 protein 60.26 6.91 At3g09260 46%
    68b 63.47 6.56 Glycosyl hydrolase family 1 protein 60.26 6.91 At3g09260 27%
    564c 87.84 6.40 Cucumisin-like Ser protease 78.28 5.91 At5g67360 14%
Seed storage proteins
    69b 43.67 6.29 12S seed storage protein (precursor) 48.03 6.56 At1g03880 18%
    70b 49.43 7.19 12S seed storage protein (precursor) 52.59 7.68 At5g44120 22%
    71b 50.44 7.67 12S seed storage protein (precursor) 52.59 7.68 At5g44120 34%
    78b 27.26 6.61 12S seed storage protein (precursor) 52.59 7.68 At5g44120 13%
    110b 57.97 6.40 12S seed storage protein (precursor) 55.86 6.36 At4g28520 19%
    245b 27.22 6.64 12S seed storage protein (precursor) 50.61 6.77 At1g03880 25%
    271c 59.96 6.52 12S seed storage protein (precursor) 55.86 6.36 At4g28520 9%
    80b 32.64 5.85 α-Cruciferin 12S (seed storage protein fragment) 34.68 6.42 At4g28520 33%
    83b 34.35 6.42 α-Cruciferin 12S (seed storage protein fragment) 34.68 6.42 At4g28520 33%
    84b 30.46 6.61 α-Cruciferin 12S (seed storage protein fragment) 31.75 6.49 At5g44120 42%
    85b 27.20 6.50 α-Cruciferin 12S (seed storage protein fragment) 27.24 6.34 At1g03880 32%
    97b 28.89 7.90 α-Cruciferin 12S (seed storage protein fragment) 31.75 6.49 At5g44120 40%
    159b 23.54 5.33 α-Cruciferin 12S (seed storage protein fragment) 27.24 6.34 At4g28520 15%
    241b 34.11 5.87 α-Cruciferin 12S (seed storage protein fragment) 34.68 6.42 At4g28520 32%
    392c 13.31 5.89 α-Cruciferin 12S (seed storage protein fragment) 31.75 6.49 At5g44120 14%
    739c 31.04 5.81 α-Cruciferin 12S (seed storage protein fragment) 31.75 6.49 At5g44120 47%
    1,154c 11.78 9.05 α-Cruciferin 12S (seed storage protein fragment) 31.75 6.49 At5g44120 5%
    1,155c 10.43 9.02 α-Cruciferin 12S (seed storage protein fragment) 31.64 9.80 At5g44120 17%
    25b 23.32 6.89 β-Cruciferin 12S (seed storage protein fragment) 21.20 6.19 At4g28520 27%
    44b 16.14 7.42 β-Cruciferin 12S (seed storage protein fragment) 20.80 7.03 At1g03880 7%
    87b 18.43 6.36 β-Cruciferin 12S (seed storage protein fragment) 20.80 7.03 At1g03880 33%
    88b 22.82 8.68 β-Cruciferin 12S (seed storage protein fragment) 21.20 6.19 At4g28520 44%
    287b 23.44 5.58 β-Cruciferin 12S (seed storage protein fragment) 20.84 9.06 At5g44120 22%
    705b 11.75 8.90 β-Cruciferin 12S (seed storage protein fragment) 21.20 6.19 At1g03880 44%
    1,153c 11.51 9.02 β-Cruciferin 12S (seed storage protein fragment) 21.20 6.19 At4g28520 39%
    1,156c 10.68 9.05 β-Cruciferin 12S (seed storage protein fragment) 20.84 9.06 At5g44120 41%
    246b 29.45 6.70 Storage proteins 7S 55.05 7.15 At3g22640 28%
    247b 34.02 6.77 Storage proteins 7S 55.05 7.15 At3g22640 18%
a

Protein numbering following Arabidopsis seed protein reference maps available at http://www.seed-proteome.com.

b

Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005).

c

Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table S2.