Figure 4.

Western-blot analysis of proteins involved in photosynthesis of B. sinuspersici that were isolated at different stages of leaf development from plants grown under ML (400 μmol quanta m⁻² s⁻¹). Western blots were obtained using antibodies against PPDK, PEPC, αNAD-ME, Rubisco SSU (solid), Rubisco LSU (hatched), ALA-AT, mASP-AT, cASP-AT, AGPase LSU, GDC-P, SHMT, tubulin, and actin. Twenty micrograms of total soluble protein was added per lane, except for Rubisco LSU, for which 5 μg was loaded per lane. Molecular masses of the immunoreactive bands are shown on the right and expressed in kilodaltons. The quantification of the immunoreactive bands is expressed as a percentage of the amount in M leaves and shown below each western blot (n = 2 or 3). sd values are shown.