Figure 6.
Cold inactivation of M. × giganteus recombinant PPDK at varying enzyme concentrations. The purified recombinant M. × giganteus PPDKs were diluted to varying protein concentrations and incubated at 0°C for the time indicated. The specific PPDK activities immediately before the cold treatment at time 0 were assigned as 100%. The data were fitted to a first-order exponential decay (Origin-Pro 7.5, OriginLab). The t1/2 (the time points at which PPDK activity fell to 50% of the initial value) at the different protein concentrations are shown.