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. 2008 Sep;148(1):304–315. doi: 10.1104/pp.108.123331

Figure 5.

Figure 5.

COR, PLDα1, and PLDδ expression in wild-type (WT), acbp6 mutant, and ACBP6 overexpressor (OE-3 and OE-5) plants. A, Northern-blot analysis using digoxigenin-labeled COR47, COR6.6, COR78, and COR15a PCR-generated cDNA probes. The membrane was subsequently stripped and hybridized to digoxigenin-labeled ACBP6 cDNA probe. Total RNA was extracted from rosettes of wild-type, acbp6, and transgenic Arabidopsis before (NA) and after (CA) cold acclimation for 3 d. The bottom panel shows total RNA (30 μg lane−1) stained with ethidium bromide. B, Northern-blot analysis of PLDα1 and PLDδ expression in wild-type, acbp6 mutant, and ACBP6 overexpressor (OE-3 and OE-5) plants. Total RNA (30 μg lane−1) was extracted from rosettes of wild-type, acbp6, OE-3, and OE-5 plants harvested before (NA) or after (CA) 3 d of cold acclimation, followed by freezing at −8°C for 1 h (F) and thawing at 4°C for 8 h (T).