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Inducibility of genes in the TNF locus by mitogen stimulation. Inducibility of NFKBIL1, LTA, TNF and LTB is shown as the ratio of transcript abundance in unstimulated cells versus cells induced with a combination of PMA and ionomycin for 6 h. Transcript levels were assayed using gene-specific primers normalized to an ACTB control primer set by real time Q-PCR using the ΔΔCt method. Mean ± SD of three replicates are shown.
