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. 2008 Jul 24;36(15):4845–4862. doi: 10.1093/nar/gkn444

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — DNase I footprinting analysis of DHS 44500. DNA probes spanning DHS 44500 radiolabelled on either the forward (panel A) or reverse (panel B) strands were digested with DNase I either alone or in the presence of NEs from Jurkat T cells. Maxam Gilbert sequencing ladder (lanes 1 and 5); naked DNA (0.0075 U DNase I) (lanes 2 and 6); NEs from unstimulated cells (0.25 U DNase I) (open circle) (lanes 3 and 7) or cells induced with PMA and ionomycin for 2 h (0.25 U DNase I) (filled circle) (lanes 4 and 8). (C) Schematic showing sites of protection in context of human and murine sequences.