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. 2008 Jul 28;36(15):5061–5073. doi: 10.1093/nar/gkn483

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — DNA-binding properties of PfHU_p. (A) EMSA depicting binding of PfHU_p to supercoiled and linear pBR322. Four hundred nanograms of supercoiled (lanes 2–6) or linear (lanes 7–11) plasmid was incubated with PfHU_p at different protein/DNA mass ratios. M, DNA marker. (B) EMSA with increasing concentrations of PfHU_p (lanes 2–7) and PfHU_Δ_C (lanes 8–13) using supercoiled pBR322 DNA. Lane 1 is free DNA. (C) Binding of PfHU_p to supercoiled DNA in the presence of 50 mM (i) and 100 mM (ii) NaCl. (D) DNA supercoiling assay with increasing concentrations of PfHU_p (lanes 3–7) and PfHU_Δ_C (lanes 8–12). Lane 1 is naked DNA (negatively supercoiled pBR322), lane 2 is pBR322 partially relaxed with topoisomerase I, lane 12 is linearized pBR322. Form-I, supercoiled DNA; form-II, relaxed DNA.