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Binding site length and affinity of PfHUp for DNA. (A) EMSA showing binding of PfHUp with end-labeled 55 bp probe. (B) EMSA of PfHUp with a 30 bp probe. (C) The Kd of the single complex obtained in (B) was determined from its binding isotherm by curve-fitting using nonlinear regression. The mean Kd value from three repeat experiments was estimated as 62.7 ± 6.5 nM.
