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. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Mol Microbiol. 2001 Feb;39(3):781–791. doi: 10.1046/j.1365-2958.2001.02268.x

Fig. 1.

Fig. 1

Phenotypes of the S. typhimurium flgN and fliT null mutants.

A. Swimming and swarming motility (assessed on 0.3% and 0.6% LB agar respectively) of WT S. typhimurium SJW1103 and the derived flgN and fliT mutants. Stationary phase broth cultures were centrally inoculated onto the agar plates and incubated at 37°C for 16 h.

B. Flagellar axial proteins in the supernatant (snt) and total cell lysate (cell) from late exponential phase cultures of WT and the flgN and fliT mutants were harvested and separated by SDS–(12%)PAGE. They were detected by immunoblotting with anti-FlgK, anti-FlgL and anti-FliD antisera or by Coomassie blue staining (FliC).