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. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Mol Microbiol. 2001 Feb;39(3):781–791. doi: 10.1046/j.1365-2958.2001.02268.x

Fig. 4.

Fig. 4

In vitro complex formation of FlgN and FliT with substrate HAPs. Gel filtration chromatography of the following purified chaperone and HAP substrate proteins, either alone or after coincubation at a 1:2 molar ratio (substrate–chaperone). Elution fractions from a calibrated Sephacryl S200-HR column were separated by SDS–(15%)PAGE and immunoblotted as indicated. Molecular weight markers are in kDa (see Fig. 2); V0, column void volume.

A. (i) His(6)FlgN alone (−K) and after incubation with His(6)FlgK (+K).Immunoblotted with anti-FlgN antiserum.

(ii) His(6)FlgK alone (−N) and after incubation with His(6)FlgN (+N). [A(ii) bottom shows the same fractions as A(i) bottom]. Immunoblotted with anti-FlgK antiserum.

B. (i) His(6)FliT alone (−D) and after incubation with His(6)FliD (+D). Immunoblotted with anti-FliT antiserum.

(ii) His(6)FliD alone (−T) and after incubation with His(6)FliD (+T). [B(ii) bottom shows the same fractions as B(i) bottom]. Immunoblotted with anti-FliD antiserum.