Fig. 5.

Recovery of ΔflhE swarming and changes in ΔflhE mutant colony morphology. (a) The ΔflhE mutant incubated (14 h) on swarm agar with and without Tween 80 (0.02% w/v) surfactant. (b) Wild-type (WT), the ΔflhE mutant and the ΔflhE mutant carrying pBAD18-FlhE were grown on agar containing calcoflour (CF; 200 μg ml^-1) for 72 h, agar containing Congo red (40 μg ml^-1) and Coomassie blue (20 μg ml^-1) (CR) for 96 h, and in LB in PVC wells for 16 h before visualization of the biofilm with crystal violet (1%).