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. 2007 Feb 7;1:175–183.

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© 2006 by the authors

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Tandem mass spectrometry identification of vimentin. The MS/MS spectrum of vimentin (obtained after trypsin digestion) is shown by analysis with ESI-Q-TOF, coupled with nanoflow capillary high-performance liquid chromatography. The precursor ion shown in the figure is m/z 648.3487, and resultant peaks were searched against the non-redundant SwissProt protein sequence database using the ProteinLynx global server. A total of twelve tryptic peptides, as shown, matched the vimentin protein.