Figure 4.

Vimentin protein levels measured in human tumors and tumor cell lines. 2-D gels were prepared using solubilized proteins from a variety of human tumors and tumor cell lines, as described in “Materials and Methods”. Background corrected integrated intensity (volume) was measured (Visage software, Genomic Solutions, Ann Arbor, MI) for total vimentin. Bars represents the average intensities for 38 lung tumors, 6 pancreatic tumors, 7 colon tumors, 25 ovarian tumors, 4 lung cell lines, 6 pancreatic cell lines, 9 colon cell lines and 33 ovarian cell lines.