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T. cruzi calreticulin binds monoglucosylated oligosaccharides. A mixture of 14C-labeled oligosaccharides were mixed with calreticulin-iminodiacetic acid-agarose beads. Applied (A), unbound (B), and bound (C; but liberated by α-methylglucoside and α-methylmannoside) compounds were run on paper chromatography. Standards: 3, Glc3Man9GlcNAc; 2, Glc2Man9GlcNAc; 1, Glc1Man9GlcNAc; 9, Man9GlcNAc.
