Figure 8.

Effect of Endo H treatment of immunoprecipitates. (A) Cells were treated as in Figure 5, but immunoprecipitates obtained with anti-calreticulin serum were treated with Endo H. (B) Immunoprecipitates obtained from cells labeled in the presence of DNJ were treated with Endo H in the presence of 0.5% SDS, and the whole samples (not only material bound to the Sepharose beads) were run on 10% SDS-PAGE and submitted to autoradiography. (C) Anti-cruzipain serum was added to supernatants of Endo H-treated 0-, 5-, and 10-min chase (+DNJ) samples (A, +DNJ), and immunoprecipitates were run on 10% SDS-PAGE. Molecular masses (in kilodaltons) are indicated on the left.