Figure 10.

Interaction of DdLim with rac1-GST fusion proteins. Autoradiogram of a Western blot of Dictyostelium proteins that associate with a GST-, Dictyostelium rac1A-, and human rac1-affinity resin probed with ¹²⁵I-labeled anti-Lim antibodies. DdLim present in the cell lysate was recovered mainly with activated human rac1, and to a lesser extent with Dictyostelium rac1A. No interaction of DdLim was found with a GST-control-resin. The binding of DdLim to both rac1-proteins was about 5-fold more in the prescence of rac1 charged with GTPγS compared with GDP.