Abstract
With the aim of illustrating a mechanism of cytomegalovirus (CMV) venereal transmission, we induced murine CMV infection in the mouse testes of immunologically competent mice. Using in situ cytohybridization, we were able to show that murine CMV-specific DNA was associated with spermatocytes and mature sperm. Electron microscopy studies also supported sperm infection. The virus could be reisolated from infected epididymal sperm by cocultivation with mouse embryo fibroblasts. We found no difference in either the sexual performance or the fertilization efficiency of the sperm between infected and uninfected males.
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- Baskar J. F., Huang E. S. Effect of murine cytomegalovirus on implantation stage mouse embryos. Proc Soc Exp Biol Med. 1981 Sep;167(4):581–587. doi: 10.3181/00379727-167-41219. [DOI] [PubMed] [Google Scholar]
- Baskar J. F., Stanat S. C., Huang E. S. Cytomegalovirus infection of murine testicular interstitial Leydig cells. Infect Immun. 1983 May;40(2):726–732. doi: 10.1128/iai.40.2.726-732.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baskar J. F., Stanat S. C., Sulik K. K., Huang E. S. Murine cytomegalovirus-induced congenital defects and fetal maldevelopment. J Infect Dis. 1983 Nov;148(5):836–843. doi: 10.1093/infdis/148.5.836. [DOI] [PubMed] [Google Scholar]
- Biggar R. J., Andersen H. K., Ebbesen P., Melbye M., Goedert J. J., Mann D. L., Strong D. M. Seminal fluid excretion of cytomegalovirus related to immunosuppression in homosexual men. Br Med J (Clin Res Ed) 1983 Jun 25;286(6383):2010–2012. doi: 10.1136/bmj.286.6383.2010. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brautigam A. R., Oldstone M. B. Replication of murine cytomegalovirus in reproductive tissues. Am J Pathol. 1980 Jan;98(1):213–224. [PMC free article] [PubMed] [Google Scholar]
- Chretien J. H., McGinniss C. G., Muller A. Venereal causes of cytomegalovirus mononucleosis. JAMA. 1977 Oct 10;238(15):1644–1645. [PubMed] [Google Scholar]
- Dutko F. J., Oldstone M. B. Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. J Exp Med. 1981 Nov 1;154(5):1636–1651. doi: 10.1084/jem.154.5.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dutko F. J., Oldstone M. B. Murine cytomegalovirus infects spermatogenic cells. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2988–2991. doi: 10.1073/pnas.76.6.2988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanshaw J. B. Congenital and acquired cytomegalovirus infection. Pediatr Clin North Am. 1966 May;13(2):279–293. doi: 10.1016/s0031-3955(16)31838-7. [DOI] [PubMed] [Google Scholar]
- Huang E. S., Chen S. T., Pagano J. S. Human cytomegalovirus. I. Purification and characterization of viral DNA. J Virol. 1973 Dec;12(6):1473–1481. doi: 10.1128/jvi.12.6.1473-1481.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jordan M. C., Rousseau W. E., Noble G. R., Steward J. A., Chin T. D. Association of cervical cytomegaloviruses with venereal disease. N Engl J Med. 1973 May 3;288(18):932–934. doi: 10.1056/NEJM197305032881803. [DOI] [PubMed] [Google Scholar]
- Lang D. J., Kummer J. F. Cytomegalovirus in semen: observations in selected populations. J Infect Dis. 1975 Oct;132(4):472–473. doi: 10.1093/infdis/132.4.472. [DOI] [PubMed] [Google Scholar]
- Lang D. J., Kummer J. F. Demonstration of cytomegalovirus in semen. N Engl J Med. 1972 Oct 12;287(15):756–758. doi: 10.1056/NEJM197210122871508. [DOI] [PubMed] [Google Scholar]
- Martin G. R. Teratocarcinomas as a model system for the study of embryogenesis and neoplasia. Cell. 1975 Jul;5(3):229–243. doi: 10.1016/0092-8674(75)90098-7. [DOI] [PubMed] [Google Scholar]
- Osborn J. E., Walker D. L. Virulence and attenuation of murine cytomegalovirus. Infect Immun. 1971 Feb;3(2):228–236. doi: 10.1128/iai.3.2.228-236.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pagano J. S. Diseases and mechanisms of persistent DNA virus infection: latency and cellular transformation. J Infect Dis. 1975 Aug;132(2):209–223. doi: 10.1093/infdis/132.2.209. [DOI] [PubMed] [Google Scholar]
- Papaioannou V. E., McBurney M. W., Gardner R. L., Evans M. J. Fate of teratocarcinoma cells injected into early mouse embryos. Nature. 1975 Nov 6;258(5530):70–73. doi: 10.1038/258070a0. [DOI] [PubMed] [Google Scholar]
- Sever J., White L. R. Intrauterine viral infections. Annu Rev Med. 1968;19:471–486. doi: 10.1146/annurev.me.19.020168.002351. [DOI] [PubMed] [Google Scholar]
- Weller T. H. The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. I. N Engl J Med. 1971 Jul 22;285(4):203–214. doi: 10.1056/NEJM197107222850406. [DOI] [PubMed] [Google Scholar]
- Willmott F. E. Cytomegalovirus in female patients attending a VD clinic. Br J Vener Dis. 1975 Aug;51(4):278–280. doi: 10.1136/sti.51.4.278. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young J. A., Cheung K. S., Lang D. J. Infection and fertilization of mice after artificial insemination with a mixture of sperm and murine cytomegalovirus. J Infect Dis. 1977 May;135(5):837–840. doi: 10.1093/infdis/135.5.837. [DOI] [PubMed] [Google Scholar]