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. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Biochemistry. 2006 Dec 6;45(51):15310–15317. doi: 10.1021/bi0614939

Figure 1. Residues targeted for Cys scanning mutagenesis in Ste2.

Figure 1

90 different mutants were constructed in which one of the shaded residues was substituted with Cys. This Ste2 topology represents the working model at the start of these studies. Circled residues indicate the positions of Cys substitutions that caused defects in halo assays for cell division arrest.