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. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Biochemistry. 2006 Dec 6;45(51):15310–15317. doi: 10.1021/bi0614939

Figure 4.

Figure 4

MTSEA-biotin reactivity of Cys residues substituted across intracellular loop 1. Assays were carried out by treating with MTSEA-biotin, solubilizing in detergent buffer, and then immunoprecipating HA-tagged Ste2 with anti-HA immobilized beads. Degree of receptor reactivity with MTSEA-biotin was quantified on Western blots probed with anti-HA to detect Ste2, and with Streptavidin to detect biotinylated Ste2. In each set of assays, control samples showed that the wild-type Ste2 lacking Cys residues did not react significantly, and that the Ste2-A299C mutant reacted well with MTSEA-biotin. The results for the mutants were normalized to the reactivity of Ste2-A299C, and represent the average of two to five independent assays.