Skip to main content
. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Biochemistry. 2006 May 16;45(19):6003–6011. doi: 10.1021/bi052247k

FIGURE 2.

FIGURE 2

Electronic absorbance spectra of pure Ctb. All spectra were scanned against a 50 mM Tris HCl pH 7.0 baseline and are normalised to heme concentration (0.001 nM). Native Ctb corresponds to the globin as it elutes from the gel filtration column (peak maxima at 411, 512, 542, 578 and 640 nm). The other forms of Ctb were generated as described in the text. Peak maxima are as follows. Fe2+, 432 and 565 nm; Fe3+, 410, 512, 542, 582 and 640 nm; Fe2+-CO, 421, 538 and 569 nm; Fe2+-O2, 414, 542 and 578 nm.