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. 2008 Sep 8;182(5):873–883. doi: 10.1083/jcb.200801054

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© 2008 Queralt and Uhlmann

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Zds1 is required for separase-induced Cdc14 early anaphase release. (A) Zds1 and Zds2 are required for separase-induced Cdc14 release. Strains Y1748 (MATa GAL-ESP1-HA CDC14-Pk₉), Y1790 (as Y1748, but zds1Δ), Y1959 (as Y1748, but zds2Δ), and Y2189 (as Y1790, but zds1Δ zds2Δ) were arrested in metaphase by nocodazole treatment and separase expression induced. Separase levels were analyzed by Western blotting, using Cdc28 as loading control. (B) Separase, Slk19, and Spo12 are not required for Zds1-induced Cdc14 release. Strains Y3288 (MATa MET-CDC20 GAL-Flag-ZDS1 CDC14-Pk₉) and Y1430 (as Y3288, but esp1-2) were arrested in metaphase by Cdc20 depletion at 23°C and shifted to 37°C for 60 min before Zds1 induction. Strains Y3288 (MATa MET-CDC20 GAL-Flag-ZDS1 CDC14-Pk₉), Y1521 (as Y3288, but slk19Δ), and Y1646 (as Y3288, but spo12Δ) were arrested in metaphase by Cdc20 depletion at 23°C and Zds1 expression was induced.