Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Sep 8;182(5):873–883. doi: 10.1083/jcb.200801054

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Queralt and Uhlmann

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Zds1 promotes PP2A^Cdc55 down-regulation. (A) Zds1 causes down-regulation of PP2A^Cdc55 phosphatase activity. Strains Y2627 (MATa MET-CDC20 HA₃-CDC55 CDC14-myc₁₈) and Y1015 (as Y2627, but GAL-Flag-ZDS1 CDC14-Pk₉) were arrested in metaphase by Cdc20 depletion and galactose added to induce Zds1 expression. The specific phosphatase activity of immunopurified Cdc55 was measured as described in Materials and methods. Mean and standard deviation of the specific phosphatase activity relative to metaphase in three experiments are shown. Cdc14 nucleolar release in these experiments was analyzed in parallel. (B) Zds1 and Zds2 are dispensable for early Cdc14 nucleolar release in the absence of Cdc55. Strains Y2831 (MATa cdc55Δ CDC14-Pk₉) and Y3419 (as 2831, but zds1Δ zds2Δ) were synchronized in G1 by pheromone α-factor treatment and released to progress through the cell cycle into nocodazole-imposed metaphase arrest. The budding index and Cdc14 nucleolar release were determined.