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. 2008 Sep 8;182(5):937–950. doi: 10.1083/jcb.200801152

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© 2008 Claypool et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — 2D BN/SDS-PAGE analysis of the AAC2 interactome with and without CL. (A–C) After CNAP, the final concentrated eluates were resolved in the first dimension by 6–16% BN-PAGE and in the second dimension by 12–18% SDS-PAGE. SYPRO Ruby detected bands were extracted, digested with trypsin, and proteins identified by LC–MS/MS. Respiratory complex III and IV subunits are labeled in red and blue, respectively, and members of the mitochondrial carrier family in purple. The pink circle highlights contaminating catalase (verified by LC–MS/MS) from the BN-PAGE molecular weight markers. (D–F) After CNAP, the final concentrated eluates were resolved by 2D BN/SDS-PAGE and immunoblotted for the indicated mitochondrial proteins. n = 3. The sources of the purified extracts were (A and D) Δaac2[AAC2], (B and E) Δaac2[CNAPAAC2], and (C and F) Δaac2Δcrd1[CNAPAAC2].