Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Maturation of SNAP-25 into the palmitoylated form occurs within 20 min of its synthesis. PC12 cells were metabolically labeled with [35S]methionine for various times. Cell lysates were either collected immediately (lanes 1–5) or collected following a chase period of 2 h (lane 6). SNAP-25 was immunoprecipitated and the state of palmitoylation was assessed by the mobility of the protein on SDS-PAGE.
