Figure 3.

Only the palmitoylated form of SNAP-25 associates with membranes. PC12 cells were metabolically labeled with [³⁵S]methionine or [³H]palmitate for 1 h, followed by homogenization and separation into particulate and soluble fractions by high-speed centrifugation. SNAP-25 was immunoprecipitated from the total cell lysate (TCL), particulate fractions (P100), and soluble fractions (S100). The presence of radiolabeled SNAP-25 in each of the fractions was evaluated by SDS-PAGE and fluorography.