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PSN375963 and PSN632408 have opposite effects on [Ca2+]i in MIN6c4 cells. MIN6c4 cells were incubated in HEPES–Krebs–Ringer bicarbonate buffer with 2.8 mM glucose for 30 min. Cells were subsequently maintained at 2.8 mM glucose or brought to 16 mM glucose followed by the addition of the indicated concentrations of PSN375963 (a and b) or PSN632408 (c and d). [Ca2+]i was continuously monitored by FLIPR.
