Table 1.

Partial list of recently established Hsp90 in vitro client proteins

Protein	Function
Smyd2	catalyzes methylation of p53 (K370) and histone H3 (K36) [45, 48]
Smyd3	catalyzes methylation of histone H3 (K4) [47]
RNA polymerase II	RNA polymerase [46]
FKBP38	calcineurin inhibitor [49]
MICAL	cytoskeletal regulation [50]
skNAC	muscle-specific transcription factor [51]
Bcl2	regulator of apoptosis [52]

Hsp90 stabilizes over 100 client proteins [5]. Proteins shown in this table are recently established in vitro clients that we have observed in our own laboratory, based on their enhanced solubility and yields in the Hsp90 over-expressing strains of E. coli. Figure 2 illustrates representative results.