Figure 5.
3-Cl-AHPC analogues 31 and 43 inhibit the activity of SHP-2 and CD45 protein tyrosine phosphatases in cleaving 6,8-difluoro-4-methylumbelliferyl phosphate (100 µM) as measured by fluorescence spectrometry of the cleavage product as described in the Experimental Section. SHP-2 and CD45 concentrations were 5 nM and 1 nM, respectively. Tetrazole 31, solid diamond; thiazolidinedione 43, solid square. Results shown are the average of duplicates ± SE.