Figure 8.

NLS-Spc1myc, NLS-Spc1-AYmyc, NLS-Spc1-TFmyc, and NLS-Spc1-AFmyc are imported and exported from the nucleus in the same way. (A) Wild-type cells were transformed with the pREP41-NLS-spc1⁺-myc, pREP41-NLS-spc1⁺-AY-myc, pREP41-spc1⁺-TF-myc, or pREP41-spc1⁺-AF-myc. Cells were grown in EMM₂ without thiamine to allow expression of the constructs from the nmt41 promoter. After incubation in YES medium for 4 h, the cells were stressed with 0.6 M KCl, harvested at the indicated time, and processed for immunofluorescence analysis. The localization of the proteins was assessed with antibodies to myc. (B) crm1–809 cells were transformed with the same vectors and treated as described in A.