Figure 6. Chop-null mutation protects from oxidative stress.

(A–C) Oxidized proteins (carbonyls) and lipids (HODEs). (A) Direct analysis of islets isolated from mice of the indicated genotypes; n = 2–4 mice per condition. (B and C) Oxidation products measured in islets incubated in vitro. Chop^+/+ or Chop^–/– islets were isolated, cultured overnight, and incubated with control media or media containing tunicamycin (2 μg/ml) for 10 hours (B) or 176 μM H₂O₂ for 7 hours (C). *P < 0.05; **P < 0.01; ***P < 0.001.