. Author manuscript; available in PMC: 2008 Sep 4.

Published in final edited form as: Biochemistry. 2006 Dec 19;45(51):15731–15739. doi: 10.1021/bi061108f

Table 2.

Membrane Partitioning of Cytoplasmic Domains of MIRR Signaling Subunits^a

		partition coefficient K, M^-1		change in K^c phospho/non-phospho
Protein	net charge^b	POPG	1:1 POPG/POPC	change in K^c phospho/non-phospho
ζ_cyt	+5	84000	2200
phospho-ζ_cyt^d	-5.5^e	2000	50	∼ 1/40
FcεRIγ_cyt	+3	7000	510
phospho-FcεRIγ_cyt^d	-0.5^e	1400	110	∼ 1/5
CD3ε_cyt	+11	∼ 6 × 10⁷ ^f	∼ 5 × 10⁵	-

No lipid binding was observed for CD3δ_cyt, CD3γ_cyt, Igα_cyt, and Igβ_cyt.

Using the Swiss Institute of Bioinformatics (SIB) server ExPASy (9).

Change in membrane partitioning for fully phosphorylated proteins relatively to non- phosphorylated proteins.

6 and 2 mol of phosphate/mol of protein for phospho-ζ_cyt and phospho-FcεRIγ_cyt, respectively.

Assuming pKs near 1.2 and 6.5 for phosphotyrosine phosphate group.

Estimated value.