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2-EG but not EET bound both CB1 and CB2 receptors with high affinity. A and B, both 2-11,12-EG and 2-14,15-EG competed with [3H]CP55940 (0.5 nm) for binding to rat cerebellar membranes, which express high levels of CB1 (A), and rat spleen membranes, which express high levels of CB2 (B). C and D, the parental compounds, arachidonic acid and EETs, did not inhibit [3H]CP55940 binding to CB1 receptors (C) or CB2 receptors (D).
