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2-EG concentration-dependently induced migration of HL-60 cells. HL-60 cells were treated with 100 nm 1,25-(OH)2 vitamin D3 for 5 days to induce differentiation into macrophage-like cells, which were then used for cell migration assays as described under “Experimental Procedures.” The error bars indicate S.E.; n = 4–6 separate experiments with each concentration in triplicate; *, p < 0.05 compared with control 2-AG responses at the respective concentrations indicated.
