TABLE 1.
Comparison of the Ki values of endocannabinoids
Shown are binding affinities of 2-EG and 2-AG for CB1 receptors expressed in rat cerebellar membranes and CB2 receptors expressed in rat spleen membranes. Ki values were determined using Prism 4.01 software (GraphPad) by entering the concentration of [3H]CP55940 (0.5 nm) and the characterized Kd of 2.3 nm for CB1 or 0.608 nm for CB2 (26), respectively. Results are mean ± S.E. of 5-7 independent experiments, each performed in triplicate.
|
CB ligands |
Ki |
|
|---|---|---|
| CB1 | CB2 | |
| nm | nm | |
| 2-11,12-EG | 23 ± 3 | 75 ± 6 |
| 2-14,15-EG | 40 ± 5 | 138 ± 9 |
| 2-AG | 45 ± 4 | 251 ± 15 |