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. 2008 Sep 5;283(36):24617–24627. doi: 10.1074/jbc.M803074200

FIGURE 5.

FIGURE 5.

Immunogold labeling in IMCD cells from dDAVP-treated Brattleboro rats using antibody to total AQP2 (A), anti-Ser(P)256-AQP2 (B), anti-Ser(P)264-AQP2 (C), or anti-Ser(P)269-AQP2 (D). Ser(P)269-AQP2 labeling was detected exclusively in the apical plasma membrane (apm, arrows), whereas labeling with the other antibodies as seen in both the apical plasma membrane (arrows) and intracellularly (arrowheads). For low magnification overview, scale bar, 200 nm. For the high magnification inset, scale bar, 50 nm.