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Expression of the S. cerevisiae protein kinase-encoding gene RCK1 from the regulatable nmt1 promoter in a chromosomally integrated construct causes telomere elongation in Sz. pombe. Southern blot analysis as detailed in the legend to Figure 1. Lane 1, AD1 (972h−-derived strain expressing RCK1); lane 2, 972h−; lane 3, AD2 (strain expressing RCK1; derived from PS36); lane 4, PS36 (rad1::ura4+).
