Table 2.
Semiquantitative test of growth at elevated temperature (37°C) of selected mutants, after subculturing for 100 generations
| Strain | Growth at 37°C |
|---|---|
| h− ura4-D18 leu1-32 his3 (wild-type) | +++ |
| PS32r (Δrad1) | +++ |
| KLP20 (rad1-S1) | +++ |
| GK21 (rad1-S3) | +++ |
| GK22 (rad1-S4) | +++ |
| PS56 (rad1-S56) | +++ |
| rad3-136 ura4 leu1 | + |
| NRC234 (rad3-136) | + |
| NRC3239 (rad5) | +++ |
| NRC3242 (h− rad8-190) | +++ |
| rad17-W | +++ |
| rad21-45 ura4 leu1 | + |
| h− rad26::ura4+ leu1-32 ade6-704 | ++ |
| h− chk1::ura4+ leu1-32 ade6-704 | +++ |
| hus1::LEU2 | ++ |
| cds1::ura4+ | +++ |
| PGYQ686 (Δwee1) | + |
Cells were deposited as liquid droplets on solid YPD medium and grown at 30°C or 37°C for 2 days. +++, growth at 37°C unaffected relative to 30°C; ++, slightly retarded growth at 37°C; +, markedly retarded growth.