FIGURE 6.

Kalirin9 and not Kalirin12 is involved in p75-NgR-mediated RhoA activation. A, introduction of Kalirin9 and not Kalirin12 reconstitutes a ligand-dependent RhoA activation by p75-NgR in 293T cells. The transfected 293T cells were untreated or treated with 0.3 μg/ml conjugated MAG-Fc (Ma) for 30 min, and the resulting lysates were subjected to RhoA activity assays using Rhotekin-binding protein pulldown assays. The control Western assays are also shown for each construct introduced as input. B, quantification of the RhoA-GTP levels. The RhoA-GTP levels were adjusted to the total RhoA. The increase in RhoA-GTP levels with Kalirin9 is significant compared with the control and Kalirin12 (^**, p < 0.05, Student's t test).