FIGURE 11.

The A11V mutation does not affect the rates of ATP hydrolysis and DNA strand exchange promoted by the RecA K250R protein. Reactions were carried out as described under “Experimental Procedures.” A, ATP hydrolysis promoted by RecA and RecA variants. Reactions contained 8 μm M13mp18 circular ssDNA, 5 μm RecA protein, or RecA protein mutant, 0.8 μm SSB protein, and 3 mm ATP. B, DNA strand exchange. Reactions were carried out and quantitated as described under “Experimental Procedures,” and the percent of duplex DNA present as the nicked circular product of strand exchange is plotted. Reactions contained 10 μm ϕX174 circular ssDNA, 10 μm ϕX174 linear duplex DNA, 4 μm RecA protein, or RecA protein mutant, 1 μm SSB protein, and 3 mm ATP. WT, wild type.