TABLE 1.
Rates of (d)ATP hydrolysis catalyzed by wild-type and mutant RecA proteins
RecA protein-catalyzed (d)ATP hydrolysis was monitored as described in Fig. 1. Characteristic reactions for each solution condition are those presented in Fig. 1. Rates are in units of μm/min. WT is wild type; ND is not determined.
| RecA | Poly(dT)dATP/S.D. | n | % WT | Poly(dT)ATP/S.D. | n | % WT | M13mp8 dATP/S.D. | n | % WT | M13mp8 ATP/S.D. | n | % WT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | 62.761 ± 2.583 | 29 | 100.0 | 54.804 ± 2.687 | 8 | 100.0 | 79.495 ± 2.192 | 8 | 100.0 | 63.283 ± 2.657 | 11 | 100.0 |
| K250R | 9.618 ± 0.268 | 8 | 15.3 | 7.193 ± 0.534 | 8 | 13.1 | 11.798 ± 0.195 | 4 | 14.8 | 9.126 ± 0.631 | 7 | 14.4 |
| K250N | 0.449 ± 0.044 | 7 | 0.7 | 0.289 ± 0.048 | 4 | 0.5 | 0.289 ± 0.048 | 4 | 0.4 | 0.248 ± 0.000 | 4 | 0.4 |
| K250Q | 0.434 ± 0.041 | 4 | 0.7 | 0.331 ± 0.138 | 6 | 0.6 | 0.351 ± 0.041 | 4 | 0.4 | 0.372 ± 0.045 | 6 | 0.6 |
| E96D | 1.405 ± 0.117 | 4 | 2.2 | 1.749 ± 0.062 | 6 | 3.2 | 1.384 ± 0.079 | 4 | 1.7 | 1.543 ± 0.179 | 6 | 2.1 |
| K250R/E96D | 3.515 ± 0.401 | 15 | 5.6 | 5.436 ± 0.421 | 9 | 9.9 | 2.572 ± 0.384 | 8 | 3.2 | 4.061 ± 1.175 | 7 | 6.4 |
| E96D + K250R | 20.397 ± 0.419 | 5 | 32.5 | ND | ND | ND | ||||||
| E96D + K250N | 10.165 ± 0.973 | 5 | 16.2 | ND | ND | ND | ||||||
| E96D + K250Q | 1.738 ± 0.165 | 7 | 2.8 | ND | ND | ND |
| RecA | No DNA dATP/S.D. | n | % WT | No DNA ATP/S.D. | n | % WT |
|---|---|---|---|---|---|---|
| WT | 0.744 ± 0.000 | 2 | 100.0 | 0.372 ± 0.058 | 2 | 100.0 |
| K250R | 0.661 ± 0.117 | 2 | 88.9 | 0.372 ± 0.058 | 2 | 100.0 |
| K250N | 0.331 ± 0.000 | 2 | 44.4 | 0.248 ± 0.000 | 2 | 66.7 |
| K250Q | 0.620 ± 0.175 | 2 | 83.3 | 0.413 ± 0.000 | 2 | 111.1 |
| E96D | 1.281 ± 0.292 | 2 | 172.2 | 1.818 ± 0.351 | 2 | 488.9 |
| K250R/E96D | 2.963 ± 0.263 | 7 | 398.4 | 4.339 ± 0.372 | 6 | 1166.7 |