FIGURE 10.

Substitution of the URA1 TATA box with other TATA sequences does not bypass the requirement for Mot1 in vivo. A, URA1 message levels in WT and mot1-42 strains with the AdMLP TATA box inserted in the forward (MLP-F) or reverse (MLP-R) orientation. The exact sequences of the MLP-F and MLP-R constructs are shown (upper left). URA1 RNA levels were determined by Northern blotting and were normalized to the level of ACT1 RNA in the same samples. B, quantitation of URA1 RNA in WT and mot1-42 cells harboring WT TATA sequence or the TGTAAA substitution. TGTA strains were transformed with plasmid vector or TBPm3 expression plasmid as shown. Error bars are standard deviations derived from three determinations.