Effect of silencing Nup88 expression on IMCD3 cell survival under
hypertonic conditions. A, comparison of cell growth rates of
IMCD3 clones expressing the empty vector or silencer for Nup88 under isotonic
conditions. Data indicate no significant difference in the growth rates over a
72-h period. B, comparison of cell survival and growth for empty
vector and Nup88-silenced cells under sublethal hypertonic stress conditions
(575 mosmol/kg H2O). A substantial difference in immediate cell
viability at 24 h was determined for Nup88-silenced cells as compared with
empty vector controls (42%, p < 0.001). In addition, whereas empty
vector control cells demonstrated recovery and cell growth over the ensuing 4
days, in Nup88-silenced cultures, no significant cell growth was determined
(p < 0.001 between controls and silenced cells). Data represent
the mean ± S.E. for three independent experiments performed in
triplicate (n = 9).