FIGURE 10.
Effect of silencing Nup88 expression on IMCD3 cell survival under hypertonic conditions. A, comparison of cell growth rates of IMCD3 clones expressing the empty vector or silencer for Nup88 under isotonic conditions. Data indicate no significant difference in the growth rates over a 72-h period. B, comparison of cell survival and growth for empty vector and Nup88-silenced cells under sublethal hypertonic stress conditions (575 mosmol/kg H2O). A substantial difference in immediate cell viability at 24 h was determined for Nup88-silenced cells as compared with empty vector controls (42%, p < 0.001). In addition, whereas empty vector control cells demonstrated recovery and cell growth over the ensuing 4 days, in Nup88-silenced cultures, no significant cell growth was determined (p < 0.001 between controls and silenced cells). Data represent the mean ± S.E. for three independent experiments performed in triplicate (n = 9).