FIGURE 3.

Cellular expression of Nup88 in IMCD3 cells chronically adapted to hypertonicity. Confocal immunohistochemistry analysis of IMCD3 cells grown under isotonic (left) and hypertonic (right) conditions for Nup88 protein expression (300 versus 900 mosmol/kg H₂O). Cells grown under isotonic conditions demonstrated little or no expression of Nup88 (green) as compared with cells chronically adapted to hypertonicity. Counter-staining of the nucleus with DAPI demonstrates the localization of Nup88 at the nuclear border. Scale bars, 20 μm.