Skip to main content
. 2008 Aug 22;105(35):13027–13032. doi: 10.1073/pnas.0805038105

Fig. 3.

Fig. 3.

miR-29 regulates extracellular matrix protein mRNAs. (A) Real-time PCR analysis of predicted target genes in both the borderzone and remote myocardium 3 days after MI shows a decrease in miR-29 to correlate with an increase in collagens (COL1A1, COL1A2, and COL3A1) and fibrillin (FBN1), whereas there was no significant change in elastin (ELN1). (n = 3–4 per group. BZ, borderzone. *, P < 0.05 compared with sham operated animals, + P < 0.05 compared with BZ region). (B) COS cells were transiently transfected with luciferase reporters (100 μg) linked to the 3′UTR sequences of the indicated genes. Increasing amounts of the miR-29b-1/miR-29a cluster (25–50-100 μg) repress the expression of luciferase, whereas this decrease was absent when using an unrelated miR, miR-206 (50 μg). *, P < 0.05 compared with luciferase reporter alone.