Fig. 4.
Antagonizing miR-19s and miR-181s expression in MM cell lines resulted in significant tumor suppression in nude mice. U266 and JJN3 cells (30 × 106 cells) were injected subcutaneously into the flanks of nude mice with 100 μl of matrigel solution 24 h after transfection with miR-19s and miR-181s ASOs or scrambled oligonucleotide. Mice were killed on day 35 and tumor volumes were calculated. (A) Time course of tumor growth of U266 cell line. (B) Time course of tumor growth of JJN3 cell line; tumors treated with miR-19s and miR-181s ASOs were significantly smaller than tumors of scrambled groups for both cell lines. (Scale bar, 10 mm.)