Fig. 1.

Conditional expression of Separase in FSK3 Tet-on cells develops gross aneuploidy. Development of aneuploidy in Separase-induced FSK3 Tet-on cells (clone no. 50) was assayed at 1, 2, and 5 days after doxycycline (1 μg/ml) treatment. (A) Separase protein was assayed by using Western blot (pAb, polyclonal antibody). (B–E) FACS analysis of the propidium iodide (PI) stained nuclei obtained from cells treated in the presence (Separase induced) or absence (noninduced) of doxycycline for a period of 5 and 15 days. Arrow indicates aneuploidy, shown as an additional peak. Inset shows distribution of chromosome number at that time point.