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. 2008 Aug 26;105(35):12961–12966. doi: 10.1073/pnas.0806180105

Fig. 1.

Fig. 1.

Generation of striatum-specific NR1-deficient mouse. (A) Dlx5/6-Cre-mediated lox-P recombination pattern was tested in the flacZ reporter line. Dlx5/6-Cre and fLacZ double-positive mice were subjected to X-Gal staining (Left). Magnified sections from the olfactory bulb (OB), striatum (STR), hippocampus (HP), cortex (CTX), thalamus (TH), and cerebellum (CBM) are shown (Right). Nuclei of recombination positive cells are shown in blue. (B) PCR genotyping pattern of each genotype produced. PCR product of Cre, fNR1, and wild type (WT) NR1 are 248, 398, and 400 bp, respectively. (C) Synaptic NR1 (110 kDa) expression in cortex (CTX), striatum (STR), and hippocampus (HP). Complete absence of NR1 protein expression in the mutant striatum. The same membrane filters were reblotted by using anti-β-actin antibody as a loading control (42 kDa). (D) In situ analysis of NR1 mRNA expression of the control and conditional NR1 mutant mouse brain (P16).