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. 2008 Aug 15;105(35):12831–12836. doi: 10.1073/pnas.0805898105

Fig. 3.

Fig. 3.

Phosphorimagergrams showing the polar arrest of a sliding DnaB without base pair melting by a Tus–Ter complex in the blocking but not in the non-blocking orientation. (A) Representative phosphorimagergrams of products generated by sliding DnaB at a fixed concentration (300 ng) on the 99B-54B*-45R triplex (4 fmole), in the presence of an increasing range of concentration of Tus (molar ratio 0–20) and the same on the 99NB-54NB*-45R triplex. The products are identified by the diagrams on the margins. Blocking Tus; lanes 1–3, marker DNA; lanes 4–8, substrate plus 300 ng DnaB plus Tus at molar ratios of Tus/DNA of 0, 1.6, 3.3, 6.7 and 20 molar. Non blocking Tus; lane 1, marker DNA; lanes 2 and 3, DNA plus 100 and 300 ng of DnaB, respectively; lanes 4–7, DNA plus DnaB (300 ng) plus Tus at molar ratios of, 1.6, 3.3, 6.7, and 20, respectively. B, lanes, 1 and 2, marker DNA; lanes 3–8, DNA substrate plus DnaB (300 ng), plus Tus at molar ratios of 0, 1.6, 3.3, 6.7, 6.7 and 20 respectively.